Regulatory

Part:BBa_K619897:Design

Designed by: BYU IGEM team   Group: iGEM11_BYU_Provo   (2011-09-28)


shortened prfA-UTR thermosensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

note the E. coli optimized Shine-Delgarno which is also present in the parent (BBa_K619889)


Source

Patterned after prfA-UTR thermosensor from Listeria monocytogenes but produced by designing oligos that would anneal to produce the desired double stranded DNA

References